Modified Nucleosides
5-Hydroxyuridine
Quick view. We supply 5-Hydroxyuridine as a specialty product for nucleic acid chemistry and oligonucleotide research workflows. CAS 957-77-7. Catalog target purity: Specification on request.
Product profile
5-Hydroxyuridine is classified as nucleoside or protected nucleoside intermediate (Formula C9H12N2O7; molecular weight 260.2).
5-Hydroxyuridine is a uridine, a nucleoside; widely distributed in nature. Uridine is one of the four basic components of ribonucleic acid (RNA).
For 5-Hydroxyuridine, reported solubility: Water (Slightly, Heated, Sonicated). Storage: Hygroscopic, -20°C Freezer, Under inert atmosphere.
Names and search terms
Confirmed or normalized name variants
- 4,5-Dihydroxy-1-pentofuranosylpyrimidin-2(1H)-one
- 5-Hydroxy-uridin
- Uridine, 5-hydroxy-
- 5-Hydroxyuridine, isobarbituridine
- 1-beta-D-Ribofuranosylisobarbituric acid
- 1-(β-D-Ribofuranosyl)-5-hydroxypyrimidine-2,4(1H,3H)-dione
Useful catalog search terms
- CAS 957-77-7
- Nucleoside or protected nucleoside intermediate
- fluorescent label
- Modified Nucleosides
Physicochemical data
| Key chemistry motifs | fluorescent label |
|---|---|
| InChI | InChI=1S/C9H12N2O7/c12-2-4-5(14)6(15)8(18-4)11-1-3(13)7(16)10-9(11)17/h,4-6,8,12-15H,2H2,(H,10,16,17)/t4-,5-,6-,8-/m1/s |
| InChIKey | QXDXBKZJFLRLCM-UAKXSSHOSA-N |
| SMILES | OC[C@H]1O[C@@H](N2C=C(O)C(=O)NC2=O)[C@H](O)[C@@H]1O |
| Melting point | >240°C (dec.) |
| Density | 1.848±0.06 g/cm3(Predicted) |
| Form | Solid |
| Color | White to Off-White |
Release documentation
The current specification defines the analytical method and acceptance criteria; the released lot result is reported on the CoA.
Additional HPLC, MS or NMR documentation can be agreed during technical review where available.
Additional application examples
- 5-Hydroxyuridine can serve as a starting material, protected intermediate or analytical comparator in nucleoside and nucleotide route development.
- Typical work includes protection/deprotection studies, phosphorylation or phosphitylation route scouting and impurity identification.
- The material can support medicinal-chemistry analog preparation and process-development feasibility studies.
Product-specific evaluation notes
Potential advantages
- Fluorescent labels enable non-radioactive detection and can reduce downstream staining or secondary-label steps.
Method limitations
- Dye-labeled nucleotides or amidites are bulkier than native monomers; incorporation efficiency, label density, photobleaching and light exposure should be controlled.
These points describe 5-Hydroxyuridine or its named chemical feature. Broader product-family guidance is maintained on the category page.
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Frequently asked questions
Can the package size for 5-Hydroxyuridine be customized?
Yes. Share the initial quantity, expected future scale, delivery timeline and packaging preference for review.
What information should be included in a quote request?
Quote catalog number BX-MN-0717 and CAS 957-77-7, the intended research workflow, required quantity and any additional HPLC, MS or NMR documentation needed.
Product-relevant literature
The publications below discuss 5-Hydroxyuridine or the named structural feature represented by this product.