2'-O-Me Phosphoramidites
DMTr-2'-O-Me-PseudoU-3'-CE-Phosphoramidite
Quick view. We supply DMTr-2'-O-Me-PseudoU-3'-CE-Phosphoramidite as a 2'-O-methyl pseudouridine amidite for modified RNA synthesis and pseudouridine-containing probe design. CAS 199737-09-2. Catalog target purity: ≥95%.
Product profile
DMTr-2'-O-Me-PseudoU-3'-CE-Phosphoramidite is classified as protected oligonucleotide phosphoramidite building block (Formula C40H49N4O9P; molecular weight 760.82).
DMTr-2'-O-Me-PseudoU-3'-CE-Phosphoramidite can be evaluated as a monomer or functional building block in solid-phase oligonucleotide synthesis.
For DMTr-2'-O-Me-PseudoU-3'-CE-Phosphoramidite, modification benefits are context-dependent; transcript length, substitution level, enzyme system and analytical purity can change the observed outcome.
Names and search terms
Confirmed or normalized name variants
- DMT-2'-OMe-Pseudouridine
- 2'-O-Methyl-5'-O-DMT-pseudouridine 3'-CE phosphoramidite
- DMT-2'-O-Me-PseudoU-3'-CE-Phosphoramidite
- DMTr-2'-O-Me-PseudoU-3'-2-cyanoethyl phosphoramidite
Useful catalog search terms
- CAS 199737-09-2
- Protected oligonucleotide phosphoramidite building block
- DMTr/DMT protection
- 2'-O-methyl modification
- phosphoramidite coupling group
- 2'-O-Me Phosphoramidites
Physicochemical data
| Key chemistry motifs | DMTr/DMT protection; 2'-O-methyl modification; phosphoramidite coupling group |
|---|
Release documentation
The current specification defines the analytical method and acceptance criteria; the released lot result is reported on the CoA.
- We release this product against the stated purity specification; the lot-specific CoA provides the result and analytical basis.
Additional HPLC, MS or NMR documentation can be agreed during technical review where available.
Additional application examples
- Typical development work includes coupling-condition screening, deprotection compatibility checks and LC-MS/HPLC characterization of test sequences.
- The material can also support related-analog comparison, impurity-reference preparation and route-development studies.
Product-specific evaluation notes
Potential advantages
- Base modifications can tune RNA stability, translation behavior, duplex properties or analytical discrimination versus native bases.
- 2′-O-methyl groups are widely used to increase nuclease resistance and modulate hybridization behavior.
Method limitations
- Modification benefits are context-dependent; transcript length, substitution level, enzyme system and analytical purity can change the observed outcome.
- Excessive or poorly positioned substitution can reduce desired enzyme interactions or activity in some assay formats.
These points describe DMTr-2'-O-Me-PseudoU-3'-CE-Phosphoramidite or its named chemical feature. Broader product-family guidance is maintained on the category page.
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Frequently asked questions
Can the package size for DMTr-2'-O-Me-PseudoU-3'-CE-Phosphoramidite be customized?
Yes. Share the initial quantity, expected future scale, delivery timeline and packaging preference for review.
What information should be included in a quote request?
Quote catalog number BX-2OMP-0029 and CAS 199737-09-2, the intended research workflow, required quantity and any additional HPLC, MS or NMR documentation needed.
Product-relevant literature
The publications below discuss DMTr-2'-O-Me-PseudoU-3'-CE-Phosphoramidite or the named structural feature represented by this product.