Isonucleosides
N1-Me-Pseudouridine
Quick view. We supply N1-Me-Pseudouridine as a key modified uridine nucleoside used in mRNA and RNA biology research. CAS 13860-38-3. Catalog target purity: Specification on request.
Product profile
N1-Me-Pseudouridine is classified as nucleoside or protected nucleoside intermediate (Formula C10H14N2O6; molecular weight 258.229).
N1-Me-Pseudouridine — 1-Methylpseudouridine is a substitute for uridine in modified mRNA. This substitution has shown to increase transfection by reducing immuogenicity. The 1-methylpseudouridine substituted mRNA increases protein expression due to low immune stimulation as it evades detection by the edosomal Toll-like receptor (TLR3).
For N1-Me-Pseudouridine, reported solubility: >20 mg/mL in EtOH; >20.65 mg/mL in DMSO. Storage: Store at -20°C.
Names and search terms
Confirmed or normalized name variants
- U-50228
- Antibiotic U-50228
- 1-N-ME-PSEUDOURIDINE
- 1-methylpseudouridine
- N1-Methylpseudouridine
- 2,4(1H,3H)-Pyrimidinedione,1-methyl-5-b-D-ribofuranosyl-
- 1-Methyl-5-(β-D-ribofuranosyl)-2,4(1H,3H)-pyrimidinedione
- 1-Methyl-5-(β-D-ribofuranosyl)pyrimidine-2,4(1H,3H)-dione
Useful catalog search terms
- CAS 13860-38-3
- Nucleoside or protected nucleoside intermediate
- Isonucleosides
Physicochemical data
| Density | 1.576±0.06 g/cm3(Predicted) |
|---|---|
| pKa | 9.43±0.10(Predicted) |
| Form | A solid |
| Color | White to off-white |
| SMILES | [C@@H]1(O)[C@@H](CO)O[C@@H](C2C(=O)NC(=O)N(C)C=2)[C@@H]1O |&1:0,2,6,16,r| |
| InChI | InChI=1/C10H14N2O6/c1-12-2-4(9(16)11-10(12)17)8-7(15)6(14)5(3-13)18-8/h,5-8,13-15H,3H2,1H3,(H,11,16,17)/t5-,6-,7-,8+/s |
| InChIKey | UVBYMVOUBXYSFV-CAYFCZNJNA-N |
Release documentation
The current specification defines the analytical method and acceptance criteria; the released lot result is reported on the CoA.
Additional HPLC, MS or NMR documentation can be agreed during technical review where available.
Additional application examples
- N1-Me-Pseudouridine can serve as a starting material, protected intermediate or analytical comparator in nucleoside and nucleotide route development.
- Typical work includes protection/deprotection studies, phosphorylation or phosphitylation route scouting and impurity identification.
- The material can support medicinal-chemistry analog preparation and process-development feasibility studies.
Product-specific evaluation notes
Potential advantages
- Base modifications can tune RNA stability, translation behavior, duplex properties or analytical discrimination versus native bases.
Method limitations
- Modification benefits are context-dependent; transcript length, substitution level, enzyme system and analytical purity can change the observed outcome.
These points describe N1-Me-Pseudouridine or its named chemical feature. Broader product-family guidance is maintained on the category page.
Related products
Frequently asked questions
Can the package size for N1-Me-Pseudouridine be customized?
Yes. Share the initial quantity, expected future scale, delivery timeline and packaging preference for review.
What information should be included in a quote request?
Quote catalog number BX-MN-0177 and CAS 13860-38-3, the intended research workflow, required quantity and any additional HPLC, MS or NMR documentation needed.
Product-relevant literature
The publications below discuss N1-Me-Pseudouridine or the named structural feature represented by this product.