Cap Analogs
CAP m7(3'-OMeG)(5')vppp(5')(2'-OMe, m6A)pU Ammonium Salt
Quick view. CAP m7(3'-OMeG)(5')vppp(5')(2'-OMe,m6A)pU Ammonium Salt is a vinyl-phosphonate cap analog with 3'-O-methyl m7G and internal m6A/2'-OMe features for mRNA capping research. A public CAS number is not assigned for this exact form. Catalog target purity: ≥98%.
Product profile
CAP m7(3'-OMeG)(5')vppp(5')(2'-OMe, m6A)pU Ammonium Salt is classified as rna cap analog or cap-related nucleotide.
CAP m7(3'-OMeG)(5')vppp(5')(2'-OMe, m6A)pU Ammonium Salt can support enzyme-substrate, in-vitro transcription, polymerase-screening or analytical-reference studies as appropriate to its phosphate state.
For CAP m7(3'-OMeG)(5')vppp(5')(2'-OMe, m6A)pU Ammonium Salt, modification benefits are context-dependent; transcript length, substitution level, enzyme system and analytical purity can change the observed outcome.
Names and search terms
Useful catalog search terms
- RNA cap analog or cap-related nucleotide
- 2'-O-methyl modification
- Cap Analogs
Physicochemical data
| Key chemistry motifs | 2'-O-methyl modification |
|---|
Release documentation
The current specification defines the analytical method and acceptance criteria; the released lot result is reported on the CoA.
Additional HPLC, MS or NMR documentation can be agreed during technical review where available.
Additional application examples
- Typical qualification includes identity, salt/counterion, assay basis, water content and enzyme compatibility.
- Related uses include LC-MS/HPLC method development and comparison with native or modified nucleotide controls.
Product-specific evaluation notes
Potential advantages
- Base modifications can tune RNA stability, translation behavior, duplex properties or analytical discrimination versus native bases.
- 2′-O-methyl groups are widely used to increase nuclease resistance and modulate hybridization behavior.
- 3′-O-methylation provides a defined blocked ribose terminus and is analytically distinguishable from the corresponding 2′-O-methyl isomer.
- Cap analog selection can influence capping efficiency, orientation and downstream translation-related performance.
Method limitations
- Modification benefits are context-dependent; transcript length, substitution level, enzyme system and analytical purity can change the observed outcome.
- Excessive or poorly positioned substitution can reduce desired enzyme interactions or activity in some assay formats.
- The 3′-O-methyl group blocks normal 3′-hydroxyl extension; confirm that this behavior matches the intended enzyme or synthesis workflow.
- Capping outcome depends on polymerase system, cap-to-NTP ratio, transcript design and purification method.
These points describe CAP m7(3'-OMeG)(5')vppp(5')(2'-OMe, m6A)pU Ammonium Salt or its named chemical feature. Broader product-family guidance is maintained on the category page.
Related products
ARCA
Cap Analogs · CAS: 400806-46-4
BX-CA-0002CAP A(5')ppp(5')(2'-OMeA)pG Ammonium Salt
Cap Analogs · CAS: N/A
BX-CA-0003CAP 4 m7G(UNA)(5')ppp(5')(2'OMeA)pG Ammonium Salt
Cap Analogs · CAS: N/A
BX-CA-0004CAP 5 m7G(5')vppp(5')(2'OMeA)pG Ammonium Salt
Cap Analogs · CAS: 3038327-80-6
BX-CA-0005CAP 6 m7G(LNA)(5')ppp(5')(2'OMeA)pG Ammonium Salt
Cap Analogs · CAS: 2645354-92-1
BX-CA-0006CAP 7 m7G(HNA)(5')ppp(5')(2'OMeA)pG Ammonium Salt
Cap Analogs · CAS: N/A
Frequently asked questions
Can the package size for CAP m7(3'-OMeG)(5')vppp(5')(2'-OMe, m6A)pU Ammonium Salt be customized?
Yes. Share the initial quantity, expected future scale, delivery timeline and packaging preference for review.
What information should be included in a quote request?
Quote catalog number BX-CA-0019, the intended research workflow, required quantity and any additional HPLC, MS or NMR documentation needed.
Product-family literature
The publications below provide peer-reviewed context for the Cap Analogs product family and its named chemistry.